Taken together , these results indicate that PmrA functions as a negative regulator of ssaG .
In principle , PmrA could repress ssaG transcription directly , by binding to the ssaG promoter , or , indirectly , by hindering transcription of an ssaG activator or by furthering expression of an ssaG repressor .
Second , chromatin immunoprecipitation experiments revealed that an HA-tagged PmrA bound to the ssaG promoter .