In the present study , the expression from lac fusion was retained after disruption in hilD in cells , suggesting the contribution of RtsA on the expression from the hilA promoter in these mutants .
In the present study , the expression from the hilA was retained after disruption in hilD in cells , suggesting the contribution of RtsA on the expression from the hilA promoter in these mutants .
that RtsA are each capable of independently inducing expression of the hilD genes
We demonstrate that RtsA are each capable of inducing expression of hilD .
We had previously demonstrated that RtsA increased expression of hilD .
We wanted to determine if RtsA could induce expression of hilD in the absence of the other regulators .
RtsA also induced expression of hilD .
RtsA induced expression of hilD ~ 10-to 12-fold .
These data show that RtsA are each capable of independently inducing expression of hilD , consistent with our model that RtsA constitute a feed forward regulatory loop .
We show that RtsA can each independently activate expression of the hilD genes .
RtsA are clearly able to induce hilD transcription .
RtsA are all also capable of activating expression of hilD independent of each other and comprise a complex feed forward regulatory loop .