Such conditions may occur when the patho-gen invades host cells as in-vivo measurements suggest that Salmonella-containing vacuoles are low in ions found that , in contrast to the PhoPQ repression effect , RpoS stimulates the expression of flagella as reduced amounts of flagellin were found in an rpoS : : kan mutant .
Such conditions may occur when the patho-gen invades host cells as in-vivo measurements suggest that Salmonella-containing vacuoles are low in Mg2 found that , in contrast to the PhoPQ repression effect , RpoS stimulates the expression of flagella as reduced amounts of flagellin were found in an rpoS : : kan mutant .
Such conditions may occur when the patho-gen invades host cells as in-vivo measurements suggest that Salmonella-containing vacuoles are acidic in ions found that , in contrast to the PhoPQ repression effect , RpoS stimulates the expression of flagella as reduced amounts of flagellin were found in an rpoS : : kan mutant .
Such conditions may occur when the patho-gen invades host cells as in-vivo measurements suggest that Salmonella-containing vacuoles are acidic in Mg2 found that , in contrast to the PhoPQ repression effect , RpoS stimulates the expression of flagella as reduced amounts of flagellin were found in an rpoS : : kan mutant .
In all those studies , it has been shown that induction of bioluminescence can accurately report induction of the rpoS regulon and thus , induction of RpoS .
In all those studies , it has been shown that induction of bioluminescence can accurately report induction of the rpoS regulon and thus , induction of RpoS .
In all those studies , it has been shown that induction of bioluminescence can accurately report induction of the rpoS regulon and thus , induction of RpoS .
In E. coli , the genes overlap with those of the RpoS regulon although induction of rpoS itself is not enough for the increased acid resistance observed since rpoS did not protect E. coli from subsequent exposure to pH 3 .
Presumably , the posttranscriptional mechanisms responsible for regulation of RpoS protein expression were sufficiently robust to withstand our attempts at overwhelming them through ectopic transcriptional upregulation of the rpoS gene .
However , rpoS might also contribute to the virulence of this serotype because RpoS − strains of S. Typhi are less cytotoxic than RpoS + strai
H-NS-deficient mutants strongly increased RpoS due to enhanced rpoS translation .
At exponential phase , when RpoS level should be very low in strain , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in 0.3 mol/L Fig. 4A .
At exponential phase , when RpoS level should be very low in strain , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in 0.3 mol/L NaCl LB broth .
At exponential phase , when RpoS level should be very low in strain , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in no salt Fig. 4A .
At exponential phase , when RpoS level should be very low in strain , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in no salt NaCl LB broth .
At exponential phase , when RpoS level should be very low in an rpoS , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in 0.3 mol/L Fig. 4A .
At exponential phase , when RpoS level should be very low in an rpoS , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in 0.3 mol/L NaCl LB broth .
At exponential phase , when RpoS level should be very low in an rpoS , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in no salt Fig. 4A .
At exponential phase , when RpoS level should be very low in an rpoS , only a general increase of about 20 % was observed in the b-galactosidase activity of rpoS : : Apr strains grown either in no salt NaCl LB broth .
Figure 4 ( A ) Static biofilm formation of eight targeted gene Transcriptomic comparison of biofilm and planktonic-cells showed that rpoS was highly expressed in both populations , and that several RpoS-activated genes showed biofilm-specific patterns of expression .
9 100 90 8 80 70 7 6 The rpoS alleles determine the salt-inducible VBNC state in Salmonella enterica 60 50 5 4 40 30 Osmotic stress reduced the amount of RpoS protein in the three strains during the induction of the VBNC state .
9 100 90 8 80 70 7 6 The rpoS alleles determine the salt-inducible VBNC state in Salmonella enterica 60 50 5 4 40 30 Osmotic stress reduced the amount of RpoS protein in LT2 during the induction of the VBNC state .
9 100 90 8 80 70 7 6 The rpoS alleles determine the salt-inducible VBNC state in Salmonella enterica 60 50 5 4 40 30 Osmotic stress reduced the amount of RpoS protein in S. Oranienburg Sa99004 during the induction of the VBNC state .
9 100 90 8 80 70 7 6 The rpoS alleles determine the salt-inducible VBNC state in Salmonella enterica 60 50 5 4 40 30 Osmotic stress reduced the amount of RpoS protein in S. Dublin KDX1 during the induction of the VBNC state .
In this study , we investigated effect of rpoS disruption and expression on intracellular levels of RpoS during induction of VBNC state , using S. Typhimurium strain LT2 .
In this study , we investigated effect of rpoS disruption and expression on intracellular levels of RpoS during induction of VBNC state , using S. Dublin .
In this study , we investigated effect of rpoS disruption and expression on intracellular levels of RpoS during induction of VBNC state , using S. Oranienburg .
Intracellular levels of RpoS protein during the induction of 0 1 2 3 4 5 6 7 day rpoS + , log10CFU/mL rpoS + , viability ΔrpoS , viability ΔrpoS , log10CFU/mL Fig. 5 .
Intracellular levels of RpoS protein during the induction of 0 1 2 3 4 5 6 7 8 9 Incubation time rpoS + , log10CFU/mL rpoS + , viability ΔrpoS , viability ΔrpoS , log10CFU/mL Fig. 5 .
To further distinguish between the contribution of RpoS to rdar morphotype expression , we compared the phenotype of an arcZ mutant with an rpoS mutant in the MAE52 strain background .
This tolerance appeared to be reflected in the upregulation of rpoS of the RpoS regulon .
Cell lysates were isolated from strains while in exponential phase prior to measuring the length of their lag Length of lag phase during osmotic-stress was To further investigate the potential contribution of RpoS to growth during hyperosmotic-stress , a rpoS deletion mutant was generated , M-09DrpoS .
Cell lysates were isolated from strains while in stationary phase prior to measuring the length of their lag Length of lag phase during osmotic-stress was To further investigate the potential contribution of RpoS to growth during hyperosmotic-stress , a rpoS deletion mutant was generated , M-09DrpoS .
The rpoS mutation resulted in a two-to threefoldhigher level of induction of a fiveto sixfold-higher level of induction during C starvation , suggesting that RpoS is involved in the negative regulation of the stiB locus .