When Fe -- Fur is bound to fur box , gene transcription is prevented .
Consequently , a global study of the iron modulon will provide insight into the extent and nature of Fur-regulated gene expression and may offer clues to the acid-sensitive nature of fur mutants .
In view of the disparate regulation of the S. Typhimu-rium Fe storage proteins by Fur , the virulence of a fur mutant was also examined .
Since many of the genes are regulated by the global transcriptional iron regulator Fur , real-time RT-PCR was also performed for the fur gene ; a significant , 2.5-fold reduction in gene expression was observed in the wild-type strain .
The fur gene , an important regulator of the Fur operon revealed a significant increase in transcription .
Accession Log2 geometric p-value mean fold change Gene Definition/function/gene product Pathogenicity island hilD SPI-1 regulator a spaP SPI-1 surface presentation of antigens sprB SPI-1 transcriptional regulator ssaL SPI-2 secretion system apparatus protein AE008831 6.95 AE008832 1.90 2.13 E 07 0.0033 AE008831 1.99 0.0033 AE008761 1.63 0.0012 Virulence-associated fur Regulator of the Fur possibly oxygen-radicals mig5 Macrophage-inducible , a putative carbonic anhydrase , found on virulence plasmid 5.55 E 05 AE008728 2.23 Fig. 1 .
Accession Log2 geometric p-value mean fold change Gene Definition/function/gene product Pathogenicity island hilD SPI-1 regulator a spaP SPI-1 surface presentation of antigens sprB SPI-1 transcriptional regulator ssaL SPI-2 secretion system apparatus protein AE008831 6.95 AE008832 1.90 2.13 E 07 0.0033 AE008831 1.99 0.0033 AE008761 1.63 0.0012 Virulence-associated fur Regulator of the Fur a regulon
A fur mutant is unable to grow on succinate as a sole carbon source due to a lack of positive regulation of the genes cycle by Fur .
Putative regulation by Fur was evaluated by generating three isogenic S. Typhi deletion mutants of fur .