The 210 and 235 regions of the putative RpoS-regulated promoter of yciG ( yciGp1 ) are underlined .
yciF was initially identified by a screen for RpoSregulated genes and a putative RpoS-dependent promoter has been located upstream of yciG [ 39 ] .
Because yciF was identified in a screen for RpoSregulated genes , we examined transcription of yciG : : luc with and without RpoS to determine if activation of yciGFE-katN by bile was dependent upon RpoS .