also suppressed the negative effect of the AT4 region , the 72 bp gene silencer region located between the leuO gene , by delimiting the transcriptional repression by H-NS through the binding of LeuO to the promoter region
it was suggested that H-NS represses leuO expression by interacting with an AT8 region located between LeuO binding sites AT3
it was suggested that H-NS represses leuO expression by interacting with an AT8 region located between LeuO binding sites AT7
However , the LeuO protein interacts with the LeuO binding site located downstream of leuO , making a DNA loop to counteract repression by H-NS .
Interestingly , the results indicate that H-NS is a positive regulator of STY1978 ; one possibility is that in an hns mutant the levels of LeuO increase since leuO is repressed by H-NS .
the response regulator RcsB activates leuO transcription in conjunction with BglJ , counteracting H-NS repression of leuO transcription
Because both leuO and SPI-1 are repressed by H-NS , activation of leuO transcription may provide a backup mechanism for SPI-1 repression under conditions .
Because both leuO and SPI-1 are repressed by H-NS , activation of leuO transcription may provide a backup mechanism for SPI-1 repression under conditions .
However , the fact that both leuO are repressed by H-NS suggests that LeuO might provide a backup mechanism for SPI-1 repression .
LeuO is a quiescent LTTR under standard laboratory conditions because leuO transcription is repressed by H-NS .
To study LeuO-dependent regulation of SPI-1 in S. enterica serovar Typhimurium , transcription of the leuO gene was freed from H-NS repression by introducing a T-POP element upstream of leuO ( Fig .
Such hypothetical conditions can be expected to activate leuO expression because leuO transcription is also repressed by H-NS .
The expression of leuO is repressed by H-NS , although there are some stress conditions .