In this study , we elucidated the molecular mechanism of RflM-dependent repression of flhDC .
RflM functions by repression of flhDC transcription .
To test a putative link between RflM in repression of flhDC , we performed an unbiased genetic screen using random transposon mutagenesis .
The transposon mutagenesis thus provided further evidence for a functional link between RflM in repression of flhDC .
Coordinated repression of flhDC transcription by RflM .
A. Schematic regulatory model of repression of the flagellar master regulator flhDC by coordinated action of RflM .
expression of flhC-lac _ providing further evidence for a cooperative action of RflM in repression of flhDC
RflM , however , did not repress flhDC transcription in the absence of RcsB protein .