Like STM1344 , STM1697 suppresses the transcription of class 3 flagella regulon genes by binding to FlhD .
Like STM1344 , STM1697 suppresses the transcription of class 2 flagella regulon genes by binding to FlhD .
Salmonella specific STM1697 binds to FlhD , a component of FlhDC , the master transcription regulator of flagella biosynthesis .
Both STM1344 and STM1697 suppress class 3 flagellum regulon genes by binding to FlhD
Both STM1344 and STM1697 suppress transcription of class 2 by binding to FlhD
complementation experiments demonstrated that STM1697 regulates virulence phenotypes through its interaction with FlhD .
complementation experiments demonstrated that STM1697 regulates flagellar formation through its interaction with FlhD .
Gene knockout demonstrated that STM1697 regulates virulence phenotypes through its interaction with FlhD .
Gene knockout demonstrated that STM1697 regulates flagellar formation through its interaction with FlhD .
A 2.0 Å resolution STM1697 -- FlhD structure reveals that STM1697 binds the same region of FlhD as STM134 .
experiments _ using SEC to determine if STM1697 can bind FlhD C to form STM16974FlhD4C2 complexes
experiments _ using SEC to determine if STM1697 can bind FlhD C to form STM1697 FlhD C 4 2 3 4 2
Therefore , we hypothesize that , when STM1697 binds to the FlhD subunits of steric exclusion prevents-70 RNA polymerase from binding to template DNA , thus blocking transcription .
Therefore , we hypothesize that , when STM1697 binds to the FlhD subunits of the FlhD4C2 complex prevents-70 RNA polymerase from binding to template DNA , thus blocking transcription .
The STM1697 mutants were unable to bind to FlhD in-vitro , as shown above .
STM1697 then binds to the peripheral FlhD of the FlhD4C2 complex .
STM1697 then binds to the peripheral FlhD of the FlhD4C2 complex .