In vitro binding of IHF to the hilA regulatory region To further test the hypothesis that IHF interferes with H-NS-mediated silencing of hilA , we performed competitive EMSAs between IHF on the hilA promoter region .
IHF alleviates H-NS-mediated repression of hilA transcription The effect of ihB mutant alleles on hilA expression in cells growing in LB-medium suggest that the IHF protein functions as a transcriptional regulator of the hilA gene .
IHF alleviates H-NS-mediated repression of hilA transcription The effect of ihB mutant alleles on hilA expression in cells entering the stationary-phase , the effect on hilA transcription of the H-NS antagonist in double ihf hns mutants suggest that the IHF protein functions as a transcriptional regulator of the hilA gene .
IHF alleviates H-NS-mediated repression of hilA transcription The effect of ihB mutant alleles on hilA expression in cells entering the gel-shift data suggest that the IHF protein functions as a transcriptional regulator of the hilA gene .
EMSA experiments suggest that binding of IHF to the hilA regulatory region might abolish the silencing of such a promoter region .
EMSA experiments suggest that binding of IHF to the hilA regulatory region might abolish H-NS binding .