Purified RcsB-RflM complex binds with high affinity to the flhDC promoter .
We next analyzed binding of RcsB-RflM complex to the flhDC promoter in detail .
We further demonstrated that efficient binding of the RcsB-RflM complex to the flhDC promoter required the DNA-binding domain of RflM .
We thus propose that RflM enables a stable binding of a RcsB-RflM heterodimer to the flhDC promoter .
We thus propose that RflM enables a fast binding of a RcsB-RflM heterodimer to the flhDC promoter .
Schematic model of RcsB-RflM-dependent regulation of flhDC transcription .