Additive effect of STM1697 on virulence As STM1344 both work through inhibition of FlhD4C2 functionality , we investigated whether they act additively .
Additive effect of STM1697 on virulence As STM1697 both work through inhibition of FlhD4C2 functionality , we investigated whether they act additively .
Additive effect of STM1697 on rdar biofilm formation As STM1344 both work through inhibition of FlhD4C2 functionality , we investigated whether they act additively .
Additive effect of STM1697 on rdar biofilm formation As STM1697 both work through inhibition of FlhD4C2 functionality , we investigated whether they act additively .
Additive effect of STM1697 on motility As STM1344 both work through inhibition of FlhD4C2 functionality , we investigated whether they act additively .
Additive effect of STM1697 on motility As STM1697 both work through inhibition of FlhD4C2 functionality , we investigated whether they act additively .
D. Electro mobility-shift assay _ elucidating a role of STM1697 in inhibition of FlhD4C2 interaction with its target DNA
S. typhimurium UMR1 _ Having demonstrated that STM1697 inhibits motility by interacting with FlhD4C2
S. typhimurium UMR1 _ Having demonstrated that STM1697 inhibits motility by interacting with FlhD4C2
S5B ) , suggesting that STM1697 affects rdar morphotype solely through inhibition of FlhD4C2 .
As FlhD4C2 is a highly conserved protein , we hypothesize that STM1697 homologues affect motility through inhibition of FlhD4C2 functionality also in other bacteria .
Most importantly , our studies suggested that STM1697 regulates flagellar formation by inhibiting FlhD4C2 from recruiting RNA polymerase to the promoters of class II flagellar genes .
Most importantly , our studies suggested that STM1697 regulates flagellar formation by inhibiting FlhD4C2 by disturbing the interaction between DNA .
Most importantly , our studies suggested that STM1697 regulates flagellar formation by inhibiting FlhD4C2 by disturbing the interaction between FlhD4C2 .
To determine whether STM1697 inhibits the DNA-binding activity of FlhD4C2 , we performed a series of EM-SAs .
Interestingly the purified STM1697 protein did not effectively inhibit the DNA binding activity of FlhD4C2 even if the molar ratio of FlhDC was as high as 18:1 .
Interestingly the purified STM1697 protein did not effectively inhibit the DNA binding activity of FlhD4C2 even if the molar ratio of STM1697 was as high as 18:1 .