We have investigated the transcriptional regulation and the interaction of the response regulator PhoP with the promoter regions of the PhoP-activated loci phoPQ , mgtA , slyB , pmrD , pcgL , phoN , pagC , and mgtCB .
phoP and slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification and CAMP resistance , we identified Udg , EptA , ArnA ( also named PbgP3 or PmrI ) , ArnB ( PbgP1 or PmrH ) , and ArnC ( PbgP2 or PmrF ) .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified Udg , EptA , ArnA -LRB- also named PmrI -RRB- .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified Udg , EptA , ArnA -LRB- also named PbgP3 -RRB- .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified Udg , EptA , ArnA -LRB- also named PmrI -RRB- .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified Udg , EptA , ArnA -LRB- also named PbgP3 -RRB- .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified ArnB .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified ArnB .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified PmrH .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified PbgP1 .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified PmrH .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified PbgP1 .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified ArnC .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified ArnC .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified PmrF .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for CAMP resistance , we identified PbgP2 .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified PmrF .
slyB are the most conserved genes in PhoP regulons across the Enterobacteriaceae family , and SlyB can decrease PhoP protein activity .27 SlyA can enhance the overall transcription of PhoP-activated loci .22 IraP is transcriptionally activated by PhoP to stabilize another regulator RpoS .28 For the proteins in the group for LPS modification , we identified PbgP2 .
As shown in Fig. 4C , this motif is also observed in a similar position and in the same orientation in a subset of PhoP-activated genes , including phoP , mgtA , pmrD , yrbL , slyB , and orgB ( 49 ) .
Two additional PhoP-activated genes , slyB and phoN , were used as positive and negative controls , respectively , since previous experiments have shown direct interaction of PhoP with the promoter of slyB but not with the promoter of phoN ( 50 ) .
A similar motif is found in the promoters of a subset of PhoP-activated genes , including steA , phoP , mgtA , pmrD , slyB , and orgB ( Fig. 6B ) .