In vitro analyses by DNase I footprinting show that LeuO binds the hilE promoter region .
In vitro analyses by electrophoretic-mobility-shift analysis show that LeuO binds the hilE promoter region .
In vitro analyses by slot blotting show that LeuO binds the hilE promoter region .
Measurements of β-galactosidase activities of the plasmid-borne lac fusions showed that LeuO regulates expression of the P hilE promoters in Fig. 4 .
Measurements of β-galactosidase activities of the plasmid-borne lac fusions showed that LeuO regulates expression of the P hilE promoters in an independent manne .
Measurements of β-galactosidase activities of the plasmid-borne lac fusions showed that LeuO regulates expression of the P hilE promoters in Fig. 4 .
Measurements of β-galactosidase activities of the plasmid-borne lac fusions showed that LeuO regulates expression of the P hilE promoters in an independent manne .
Measurements of β-galactosidase activities of the plasmid-borne lac fusions showed that LeuO regulates expression of the P hilE promoters in Fig. 4 .
Measurements of β-galactosidase activities of the plasmid-borne lac fusions showed that LeuO regulates expression of the P hilE promoters in an independent manne .
Binding of LeuO to the hilE promoter region To test whether LeuO is able to bind the hilE promoter region , a slot blot binding assay was performed .
Binding of LeuO to the hilE promoter region To test whether LeuO is able to bind the hilE promoter region , a slot blot binding assay was performed .
Quantitative analysis of Fig. 6B indicated that , under the conditions of the assay , LeuO bound the hilE DNA fragment with an approximate Kd of 0.37 μM .
Quantitative analysis of binding indicated that , under the conditions of the assay , LeuO bound the hilE DNA fragment with an approximate Kd of 0.37 μM .