somA require the response regulator PhoP for expression To determine whether the expression of somA is regulated by transcription factors , the GFP report-ers were transduced into Salmonella strains with mutations in one of the following genes : slyA .
VirK require the response regulator PhoP for expression To determine whether the expression of somA is regulated by transcription factors , the GFP report-ers were transduced into Salmonella strains with mutations in one of the following genes : slyA .
The PhoP Protein Binds to the Promoter of the slyA Gene -- Investigation of PhoP-controlled slyA transcription has been carried out using plasmid-borne constructs harboring the slyA promoter region ( 20 ) .
The PhoP Protein Binds to the Promoter of the slyA Gene -- Investigation of PhoP-controlled slyA transcription has been carried out using plasmid-borne constructs harboring the slyA promoter region ( 20 ) .
The PhoP/PhoQ system is required for transcription from one of the promoters of the slyA gene ( 20 ) , suggesting that some of the phenotypes displayed phoP and phoQ mutants could be caused by their inability to express the SlyA protein and implying that SlyA participates in transcription of a subset of PhoP-regulated genes .
Although the mechanism remains unknown , it has been suggested that the PhoP protein regulates slyA expression indirectly because a PhoP box could not be identified in the slyA promoter region .
that the PhoP protein binds to the slyA promoter
It has been suggested that the PhoP protein regulates the slyA gene indirectly because a PhoP box could not be identified in the slyA promoter .
The PhoP protein binds to the slyA promoter .
These results indicate that the PhoP protein controls slyA transcription directly .
Although others have proposed that the PhoP protein regulates slyA transcription indirectly -LRB- i.e. by modulating the expression of another regulatory protein -RRB- , our results argue that PhoP controls slyA transcription directly -LRB- i.e. by binding to the slyA promoter .
Although others have proposed that the PhoP protein regulates slyA transcription indirectly -LRB- i.e. by modulating the expression of another regulatory protein -RRB- , our results argue that PhoP controls slyA transcription directly -LRB- i.e. by binding to the slyA promoter .
To identify the PhoP-regulated gene ( s ) responsible for motility on 0.3 % agarose low Mg2 + media , we tested the behavior of strains mutated in each of 19 different PhoP-activated genes ( mgtA , mgtB , mgtC , mig-14 , pagC , pagK , pagM , pagN , pagO , pagP , pcgL , pgtE , phoN , pmrD , rstA , slyA , ugtL , virK , and yobG ) .