Mutants identified in the MudJ transposon mutagenesis screen Tn insertion site Description Strain PM sensitive imp/surA tolB gnd gnd yibD dgoA pmrC pmrC pmrC pmrC pmrI pmrI 851 895 JSG JSG JSG JSG JSG JSG JSG JSG JSG JSG JSG JSG 1272-1290-897-899 901-918-973-1036 923 925 PmrA regulated PmrA regulated , PM sensitive PmrA-regulated genes identified in the screen in iron resistance , JSG897 ( yibD ) and JSG899 ( dgoA ) were grown on solid N-minimal-medium supplemented with 100 M FeSO4 .
Lee , H. , Hsu , F.F. , Turk , J. , and Groisman , E.A. ( 2004 ) The PmrA-regulated pmrC gene mediates phosphoethanola-mine modification of lipid-A and polymyxin resistance in Salmonella enterica .
[ 21 ] Lee , H. , Hsu , F.F. , Turk , J. and Groisman , E.A. ( 2004 ) The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and polymyxin resistance in Salmonella enterica .
pmrC , a PmrA-regulated gene necessary for the addition of pEtN to lipid-A , did not affect core pEtN addition .
Recently , the PmrA-regulated pmrC gene product was shown to mediate the addition of pEtN to the 1-position of lipid-A and affect resistance to PM ( 22 ) .
The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid a and poly-myxin resistance in Salmonella enterica .
Lee , H. , Hsu , F.F. , Turk , J. , and Groisman , E.A. ( 2004 ) The PmrA-regulated pmrC gene mediates phosphoethanola-mine modification of lipid-A and polymyxin resistance in Salmonella enterica .
Collectively , these results strongly suggest that the pmrC , ugd and pmrG genes and the pbgPE operon are the only PmrA-regulated determinants required for Fe ( III ) resistance .
pmrC mediates the PmrA-PmrB-regulated attachment of phosphoethanolamine to lipid-A ( 27 ) .
The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and poly-myxin resistance in Salmonella enterica .
68 , 6139 -- 6146 58 Lee , H. et al. ( 2004 ) The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and polymyxin resistance in Salmonella enterica .
PmrA-regulated genes described and the modifications their products mediate are : pmrHFIJKL/pmrE , 4-aminoarabinose ; cptA , heptose I phosphoethanolamine phosphotransferase ; pmrC , lipid-A phosphoethanolamine phosphotransferase ; cld , O-antigen chain length determinant ; pmrG , heptose II phosphatase .
Lee H , Hsu FF , Turk J & Groisman EA ( 2004 ) The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and polymyxin resistance in Salmonella enterica .
The absence of transcriptional linkage between the RpoN and the PmrA regulons was corroborated because no significant differences were observed when the expression of PmrAcontrolled genes ( pmrI , pmrC , pmrF , and ugd ) was compared either in a RpoN or in a 1 DrpoN strain grown in LB ( exponential or stationary-phase ) or in a low-Mg N-21 minimal-medium , which are PhoP-and PmrA-inducing conditions ( Soncini & Groisman , 1996 ) ( Fig. 3a and data not shown ) .
( a ) b-Galactosidase activities were determined for pmrI : : MudJ , pmrC : : MudJ , pmrF : : MudJ and ugd : : MudJ ( all PmrA-regulated genes ) in an rpoN ( W-1 t ) or in a DrpoN strain .
The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and poly-myxin resistance in Salmonella enterica .
After being phosphorylated by PmrB , PmrA can effectively bind to the transcriptional sites of pmrC .
The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and polymyxin resistance in Salmonella enterica .
The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and poly-myxin resistance in Salmonella enterica .
The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and polymyxin resistance in Salmonella enterica .
Importantly , the enrichment in ssrB promoter DNA was comparable to that of other known PmrA-regulated genes , including pmrC ( Fig. 4C ) .
Lee , H. , Hsu , F.F. , Turk , J , and Groisman , E.A. ( 2004 ) The PmrA-regulated pmrC gene mediates phosphoethanol-amine modification of lipid a and polymyxin resistance in Salmonella enterica .
[ 75 ] H. Lee , F.F. Hsu , J. Turk , E.A. Groisman , The PmrA-regulated pmrC gene mediates phosphoethanolamine modification of lipid-A and polymyxin resistance in Salmonella enterica , J. Bacteriol .