Other genes varied in prevalence , including trhH ( encoding pilus assembly protein ) , sirA ( two-component system with barA ) , pagK ( PhoPQ-activated protein ) , and sseK1 ( encoding putative-secreted effector protein ) .
In contrast to S. Typhimurium and S. Paratyphi B dT , no pagK ( encoding a PhoPQ-activated protein ) was detected , but stkC was present .
Furthermore , PAT DE4 was positive for the outer membrane receptor gene instead of but no signal was obtained for the islet genes htrE ( probable porin/fimbrial assembly protein ) , sopD2 ( secreted effector protein ) , srfJ ( putative virulence factor , activated by transcription factor SsrB ) and sseK2 ( translocated effector protein SSEK2 by type III secretion system ) while pagK ( PhoPQ-activated protein ) was present only in this array type .