Efforts in our laboratory to demonstrate binding of purified HilE protein to the hilA promoter have failed , however , recent work demonstrated that the HilE protein interacts with HilD , using a bacterial two-hybrid assay .
colleagues have identified HilE as a negative regulator of hilA transcription .
Jones have identified HilE as a negative regulator of hilA transcription .
In E. coli , a hilA was regulated by HilE only when HilD protein was present .
Also , when hilD is under the control of the lac promoter , hilA is still regulated by HilE .
Also , when hilD is under the control of the lac promoter , hilA is still regulated by HilE .
To determine the effects of HilE on the regulation of hilA via PhoPQ , we conducted a-galactosidase assay .
To determine the effects of HilE on the regulation of hilA via PhoPQ , we conducted lacZY expression .
Fahlen et al. identified HilE as a negative regulator of hilA transcription provided bacterial two-hybrid data .