Therefore , we compared the transcription of HilD-regulated genes in eK297R and eWT in the lon deletion background , which excludes the effect of protein stability , and found that the messenger RNA levels of HilD-regulated genes in eK297R increased dramatically compared with these in eWT ( Figure 3D ) , indicating that K297R mimicking deacetylation can effectively activate HilD-regulated genes by increasing its DNA-binding affiffinity in the lon mutant background in-vivo .
Therefore , we compared the transcription of HilD-regulated genes in eK297R and eWT in the lon deletion background , which excludes the effect of protein stability , and found that the messenger RNA levels of HilD-regulated genes in eK297R increased dramatically compared with these in eWT ( Figure 3D ) , indicating that K297R mimicking deacetylation can effectively activate HilD-regulated genes by increasing its DNA-binding affiffinity in the lon mutant background in-vivo .