GSE88980-GSM2356688-GPL17439-PMID_28526842.tsv
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SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using cufflinks v.1.3.0 PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Genome_build: NC_000913 PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Supplementary_files_format_and_content: comma-delimited text files include FPKM values for each Sample. PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA). PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics strain: K-12 MG1655 PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics genotype: WT PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR4435465 GSE88980 GSM2356688 GPL17439 PMID_28526842 WT NaCl 2 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) contact_name Donghyuk,,Kim PGCGROWTHCONDITIONS