GSE85914-GSM2287252-GPL18133-PMID_29584733.tsv 4.27 KB
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	base calls with quality metrics were generated using the HiSeq 2500 Control software PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	sequences were aligned to the parent E. coli strain genome template and transcript levels were quantified for triplicate groups of each primary deletion strain and synthetic rescue (i.e. sup) strain using Rockhopper (version 2.0.3) PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	transcript abundance was quantified using rockhopper PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Genome_build: E. coli strain BW25113 PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	RNA was isolated using standard Trizol lysis and aqueous extraction, followed by further purification using a Qiagen RNAeasy kit. PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	library construction followed Illumina manufacturer protocol for bacteria using the Nextera XT DNA Library Preparation Kit PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	cells were grown in M9 glucose (0.4% w/v) PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	Escherichia coli cell lysate PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	strain: BW25113 PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	cells were grown in M9 glucose (0.4% w/v) PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	Escherichia coli cell lysate PGCGROWTHCONDITIONS
SRR4047474	GSE85914	GSM2287252	GPL18133	PMID_29584733	∆entC parent Sample 12	Measuring gene expression profiles of E. coli synthetic rescue strains	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	contact_name	Sean,,Crosson PGCGROWTHCONDITIONS