SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	data_processing	Illumina Casava1.7 software used for basecalling. PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	data_processing	Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Escherichia coli ATCC8739 genome (GenBank CP000946.1) using Bowtie 2 (version 2.2.5) PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	data_processing	Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	data_processing	Detailed parameters for reads mapping: bowtie2 -x ATCC8739 -1 X_1.fq -2 X_2.fq --no-mixed -p 2 -S X.sam PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	data_processing	Genome_build: ASM1938v1 PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	data_processing	Supplementary_files_format_and_content: [.txt] tab-delimited text file includes RPKM values PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	extract_protocol	Total RNA was isolated by the Trizol-Phenol-Chloroform method. An additional clean-up including the on-column DNase I treatment was performed by using the RNeasy mini kit (Qiagen). PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	extract_protocol	RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	growth_protocol	Single colonies were inoculated into 15x100 mm tubes containing 4 ml LB, and grown at 30ºC and 250 rpm overnight. 100 μl seed culture was inoculated into a 100 ml flask containing 10 ml LB medium, and grown at 30ºC and 250 rpm for 5 h. PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	organism	Escherichia coli ATCC 8739 PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	source_name	IspG1 strain PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	characteristics	strain: IspG1 PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	growth_protocol	Single colonies were inoculated into 15x100 mm tubes containing 4 ml LB, and grown at 30ºC and 250 rpm overnight. 100 μl seed culture was inoculated into a 100 ml flask containing 10 ml LB medium, and grown at 30ºC and 250 rpm for 5 h. PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	organism	Escherichia coli ATCC 8739 PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	source_name	IspG1 strain PGCGROWTHCONDITIONS
SRR3825306	GSE84255	GSM2230382	GPL22139	PMID_28864262	IspG1	Balanced activation of IspG and IspH to eliminate intermediate accumulation and improve isoprenoids production in Escherichia coli	GPL22139: Illumina HiSeq 2000 (Escherichia coli ATCC 8739)	contact_name	feiyu,,fan PGCGROWTHCONDITIONS