GSE75818-GSM1968430-GPL14548-GPL21222-PMID_27198188.tsv
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SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) data_processing Sequencing reads were aligned to the mg1655 genome (NC_000913.2) or a list of the designed signal peptide fusions using bowtie 0.12.9 PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) data_processing csv files contain 16 columns of the data, each pair corresponds to the counts from the top (odd numbers) and bottom (even numbers) strand. The first pair corresponds to counts collected at 0 minutes after rifampicin addition. Similarly, the second, third, fourth, fifth, sixth, seventh, and eigth pairs correspond to counts collected at 2, 4, 6, 8, 10, 15, and 20 minutes after rifampicin addition. The ith row in each column corresponds to the number of alignments at the ith base in NC_000913.2. PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) data_processing The sigPep.csv file contains the counts for each specific signal peptide fusion to each specific test gene. Entries with SP_spkA/D/G/K represent spike in control molecules used for normalization. PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) data_processing Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) data_processing Supplementary_files_format_and_content: sam containing aligned sequences PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) extract_protocol Cells were collected as a function of time after rifampicin treatment at the specified times. RNA was extracted using the RNAsnap protocol. PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) extract_protocol DNA was removed with Dnase I. rRNA was removed with the gram-negative RiboZero kit. Libraries were prepared with the RNAUltra directional kit from New England Biolabs for total RNA sequencing. For signal peptide fusions, cDNA was constructed using primers that target the signal-peptide-coding/barcode region of the fusion RNAs. PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) growth_protocol Cells were grown to an OD_600 of 0.4 in Luria Broth Lennox PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) source_name mg1655 PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) treatment_protocol Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) characteristics genotype/variaion: lacZ PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) characteristics time point: 6 min PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) characteristics treatment: rifampicin PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) growth_protocol Cells were grown to an OD_600 of 0.4 in Luria Broth Lennox PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) source_name mg1655 PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) treatment_protocol Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS
SRR2982505 GSE75818 GSM1968430 GPL14548-GPL21222 PMID_27198188 lacZ_6min Spatial organization shapes the turnover of a bacterial transcriptome GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) contact_name Xiaowei,,Zhuang PGCGROWTHCONDITIONS