GSE67218-GSM1642594-GPL17439-PMID_.tsv
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SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Basecalls performed using CASAVA version 1.4 PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (NC_000913) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99. PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing The expression levels were based upon the RPKM metric obtained from strand-specific manner. PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Supplementary_files_format_and_content: RPKM is generated by in-house script PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol From the cells cultured in LB media at 37 ℃, total RNA was extracted using RNAsnapTM method, followed by the ethanol precipitation. rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer’s instruction (Epicentre). rRNA removal was confirmed using ExperionTM system. Subsequently, 4 µg of the purified RNA was fragmented to sizes of ~300 bp using RNA fragmentation reagent (Ambion, Grand Island, NY). PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol 50 ng of the fragmented RNA was converted to sequencing library using TruSeq® Stranded mRNA Sample Prep Kit in accordance with manufacturer’s instruction (Illumina). PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media grown at 37 ℃ with constant agitation overnight. Cultures were diluted 1:100 into fresh minimal medium and then cultured at 37 ℃ to mid-exponential phase (OD600 nm ~ 0.6). PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol No treatment PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics cell type: Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media grown at 37 ℃ with constant agitation overnight. Cultures were diluted 1:100 into fresh minimal medium and then cultured at 37 ℃ to mid-exponential phase (OD600 nm ~ 0.6). PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol No treatment PGCGROWTHCONDITIONS
SRR1927170 GSE67218 GSM1642594 GPL17439 PMID_ RNA-seq 37C LB rep2 Transcriptional expression level of E. coli at 37 ℃ in LB media GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) contact_name Byung-Kwan,,Cho PGCGROWTHCONDITIONS