GSE65711-GSM1603393-GPL16085-PMID_26279566.tsv
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SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using cufflinks v.1.3.0 PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Genome_build: NC_000913 PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Supplementary_files_format_and_content: comma-delimited text files include FPKM values for each Sample. PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) extract_protocol Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA). PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) extract_protocol RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) growth_protocol E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) source_name ΔsoxS PQ PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics strain: K-12 MG1655 PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics genotype/variation: delta-soxS PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) growth_protocol E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) source_name ΔsoxS PQ PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR1796605 GSE65711 GSM1603393 GPL16085 PMID_26279566 ΔsoxS PQ 2 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) contact_name Donghyuk,,Kim PGCGROWTHCONDITIONS