GSE64848-GSM1581588-GPL16085-PMID_29771928.tsv
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SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing The MiSeq Reporter MiSeq Reporter 2.4.60.8 was used for basecalling and demultiplexing PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing bowtie2 v2.2.3 was used for alignment PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Reads were mapped to the NC_000913.2 reference genome using the default settings in bowtie2 (Langmead and Salzberg, 2012). Datasets were quantified using cuffdiff in the cufflinks package to generate FPKM (Framents Per Kilobase per Million reads mapped) values for all genes (Trapnell et al., 2013). PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Supplementary_files_format_and_content: Processed data is presented in a cuff diff analysis format PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) extract_protocol Total RNA was depleted of ribosomal RNAs using Epicentre’s RiboZero rRNA removal kit. PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) extract_protocol rRNA depleted RNA was then primed using random hexamers and reverse transcribed using SuperScript III (Life Technologies). PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) growth_protocol Cells were grown in shake flasks to mid-exponential phase under either aerobic or anaerobic conditions depending on the sample. M9 minimal media supplement with either glucose, fructose, or glycerol as the sole carbon source was used depending on the sample. PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) source_name E. coli K12 MG1655 PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) treatment_protocol Gene expression analysis was performed using a strand-specific, paired-end RNA-seq protocol using the dUTP method (Levin et al., 2010). Total RNA was isolated and purified using the Qiagen Rneasy Kit with on-column DNase treatment. PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics strain: Crp_delAr2 PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) growth_protocol Cells were grown in shake flasks to mid-exponential phase under either aerobic or anaerobic conditions depending on the sample. M9 minimal media supplement with either glucose, fructose, or glycerol as the sole carbon source was used depending on the sample. PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) source_name E. coli K12 MG1655 PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) treatment_protocol Gene expression analysis was performed using a strand-specific, paired-end RNA-seq protocol using the dUTP method (Levin et al., 2010). Total RNA was isolated and purified using the Qiagen Rneasy Kit with on-column DNase treatment. PGCGROWTHCONDITIONS
SRR1751315SRR1751316SRR1751317 GSE64848 GSM1581588 GPL16085 PMID_29771928 RNAseq_delAr2_glycerol_NH4Cl_O2_3 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GPL16085: Illumina MiSeq (Escherichia coli) contact_name Haythem,,Latif PGCGROWTHCONDITIONS