GSE58285-GSM1405879-GPL14548-PMID_25237058.tsv
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SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Basecalls were performed using CASAVA.1.8 PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Sequence reads were trimmed for adaptor reads and mapped to E. coli K12 MG1655 genome using CLC Genomics Workbench 6.0.1 PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Genome_build: U00096.2 PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Supplementary_files_format_and_content: Processed files are in tab delimited format. Separate files are provided for the forward and reverse strands. The start and end coordinates should be the same. Each line in the data set represents the position of the first nucleotide at the 5[linebreak] end of a sequence that could be mapped to the E. coli genome. PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) extract_protocol Total RNA was isolated as desribed in Kime et al., 2008 PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) extract_protocol Libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) growth_protocol All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) source_name E. coli cell cultures PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) treatment_protocol Cultures for samples 1 and 2 were incubated at 44°C for 45 min after growth. PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics strain: BW25113 PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics analysis: in vitro PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics genotype: n/a PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics incubation time: 0 min PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) growth_protocol All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) source_name E. coli cell cultures PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) treatment_protocol Cultures for samples 1 and 2 were incubated at 44°C for 45 min after growth. PGCGROWTHCONDITIONS
SRR1363866 GSE58285 GSM1405879 GPL14548 PMID_25237058 T170V 0 min Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GPL14548: Illumina HiSeq 2000 (Escherichia coli) contact_name Kenneth,,McDowall PGCGROWTHCONDITIONS