GSE58285-GSM1405877-GPL14548-PMID_25237058.tsv 6.29 KB
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	data_processing	Basecalls were performed using CASAVA.1.8 PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	data_processing	Sequence reads were trimmed for adaptor reads and mapped to E. coli K12 MG1655 genome using CLC Genomics Workbench 6.0.1 PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	data_processing	Genome_build: U00096.2 PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	data_processing	Supplementary_files_format_and_content: Processed files are in tab delimited format. Separate files are provided for the forward and reverse strands. The start and end coordinates should be the same. Each line in the data set represents the position of the first nucleotide at the 5[linebreak] end of a sequence that could be mapped to the E. coli genome. PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	extract_protocol	Total RNA was isolated as desribed in Kime et al., 2008 PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	extract_protocol	Libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	growth_protocol	All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	source_name	E. coli cell cultures PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	treatment_protocol	Cultures for samples 1 and 2 were incubated at 44°C for 45 min after growth. PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	characteristics	strain: N3433 PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	characteristics	analysis: in vivo PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	characteristics	genotype: rne wild-type PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	characteristics	incubation time: n/a PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	growth_protocol	All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	source_name	E. coli cell cultures PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	treatment_protocol	Cultures for samples 1 and 2 were incubated at 44°C for 45 min after growth. PGCGROWTHCONDITIONS
SRR1363864	GSE58285	GSM1405877	GPL14548	PMID_25237058	rne wild-type	Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli	GPL14548: Illumina HiSeq 2000 (Escherichia coli)	contact_name	Kenneth,,McDowall PGCGROWTHCONDITIONS