GSE54900-GSM1326353-GPL17439-PMID_25222563.tsv
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SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using cufflinks v.1.3.0 PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Genome_build: ASM584v2 PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Supplementary_files_format_and_content: Comma-delimited text file includes RPKM values for each Sample. PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA). PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics genotype/variation: Δfur PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics agent: DPD PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR1168139 GSE54900 GSM1326353 GPL17439 PMID_25222563 Δfur with DPD 1 (RNA-seq) Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) contact_name Donghyuk,,Kim PGCGROWTHCONDITIONS