SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Basecalls performed using Casava versions 1.6 or 1.7. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Sequenced reads were trimmed for adaptor sequence. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Trimmed reads were sequentially aligned using Bowtie v0.12.7 to E coli rRNA and noncoding RNA allowing one mismatch. Reads aligning to any of these indices were discarded. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	The remaining reads were aligned using Bowtie v0.12.7 against E. coli MG1655 genome using parameters -v1 -m2 -k1. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Bowtie alignments against the E coli genome were converted to wiggle files. The position of each alignment is distributed into several nucleotides in the center of the footprint. For each footprint read, the center residues that are at least 10 nucleotides away from either ends were given the same score, which is weighted by the length of the fragment [Oh et al,. Cell 147, 1295 (2011)]. Scores therefore represent the number of read alignments attributed to each genomic position under each scoring scheme. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Genome_build: NC000913.2 PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Supplementary_files_format_and_content: wiggle files with two columns: first column containing chromosome positions and second column containing the number of reads mapped to the position (see publication for details). PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	Extraction was performed as described in detail previously (Li et al., 2012; Oh et al., 2011). 200 ml of cell culture was rapidly filtered at 37C by passing through a nitrocellulose filter. Cell pellets was were rapidly collected using a pre-warmed metal table crumber, flash frozen in liquid nitrogen, and combined with frozen droplets of lysis buffer. Cells and lysis buffer were pulverized in 10 ml canisters (Retsch) pre-chilled in liquid nitrogen using Qiagen TissueLyser II. Pulverized lysate was thawed on ice and clarified by centrifugation at 4C. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	Ribosome protected mRNA fragments were size selected via gel purification, and ligated to 5[linebreak] adenylated DNA oligo. After reverse transcription, the single stranded DNA was circularized, and PCR amplified [Oh et al,. Cell 147, 1295 (2011)]. More at G.W. Li, D. Burkhardt, C.A. Gross, J. S. Weissman (Cell). PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	All cultures were based on MOPS media with 0.2% glucose (Teknova), with either full supplement (Neidhardt et al., 1974). An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 2.8-liter flask at 37C with aeration (180 rpm) until OD600 reached 0.3. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	bacteria PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	strain: MG1655 PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	media: fully supplemented MOPS glucose media PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	All cultures were based on MOPS media with 0.2% glucose (Teknova), with either full supplement (Neidhardt et al., 1974). An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 2.8-liter flask at 37C with aeration (180 rpm) until OD600 reached 0.3. PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	bacteria PGCGROWTHCONDITIONS
SRR1067773SRR1067774	GSE53767	GSM1300282	GPL14548-GPL18133	PMID_24766808	mRNA-seq in rich defined media	Absolute quantification of protein production reveals principles underlying protein synthesis rates	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli)	contact_name	Gene-Wei,,Li PGCGROWTHCONDITIONS