GSE43408-GSM1217969-GPL14548-PMID_23856776.tsv
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SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing The original image data is transfered into sequence data by base calling PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to DH10B gene using CLC Genomics Workbench, default parameters PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CLC Genomics Workbench, default parameters PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Genome_build: GI:170079663/NC_010473 PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) extract_protocol mRNA of prokaryotes is enriched just by removing rRNAs from the total RNA. Adding the fragmentation buffer, the mRNA is interrupted to short fragments (about 200 bp), then the first strand cDNA is synthesized by random hexamer-primer using the mRNA fragments as templates. Buffer, dNTPs, RNase H and DNA polymerase I are added to sythsize the second strand. The double strand cDNA is purified with QiaQuick PCR extraction kit and washed with EB buffer for end repair and single nucleotide A (adenine) addition. PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) extract_protocol sequencing adaptors are ligated to the fragments. The required fragments is purified by agrose gel electrophoresis and enriched by PCR amplification. The library products are ready for sequencing analysis via Illumina HiSeq™ 2000. PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) growth_protocol 37C in LB medium under aerobic and anaerobic condition PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) source_name E.coli Top 10 PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) treatment_protocol Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture[linebreak]s instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA). PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics strain: E.coli Top 10 PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics genotype/variation: no LL37 expression (control) PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics growth condition: anaerobic PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) growth_protocol 37C in LB medium under aerobic and anaerobic condition PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) source_name E.coli Top 10 PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) treatment_protocol Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture[linebreak]s instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA). PGCGROWTHCONDITIONS
SRR958660 GSE43408 GSM1217969 GPL14548 PMID_23856776 pHerd30T-LL37 CK+ anaerobic Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GPL14548: Illumina HiSeq 2000 (Escherichia coli) contact_name WEI,,LIU PGCGROWTHCONDITIONS