GSE126710-GSM3611674-GPL26204-PMID_31208335.tsv
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SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing quality trimming (cut-off of 20) and adapter removal using cutadapt 1.15 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing generattion of reverse complement reads using FASTX 0.0.14 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing poly A-tail removal using READemption 0.4.3 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing mapping of reads to E. coli MG1655, φO104 and φPA8 genomes using READemption 0.4.3 and segemehl 0.2.0 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing gene wise quantification (quantification of the number of reads overlapping with the locations of the annotation entries) using READemption 0.4.3 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing differential gene expression analysis based on the gene wise qunatification for E. coli MG1655 annotation using READemption 0.4.3 and DESeq2 1.16.1 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing Genome_build: E. coli MG1655 (U00096.3), φPA8 (KP682374.1) and φO104 (3256115 to 3317011 from NC_018658.1) PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) data_processing Supplementary_files_format_and_content: csv file of the gene wise quantification data used for DESeq2 analysis PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) extract_protocol Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) extract_protocol cDNA libraries for the Illumina sequencing platform were constructed using NEBNext Ultra Directional RNA Library Kit following the manufacturer[linebreak]s instructions. PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) growth_protocol Overnight cultures were diluted till OD600 of 0.005 in LB medium (10 g/L tryptone, 5 g/L yeast extract, 5 g/L NaCl) and cells were grown at 37oC, 180 rpm to an OD600 of 0.4 - 0.6. PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) organism Escherichia coli K-12 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) source_name MG1655_φPA8 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) treatment_protocol After the treatment with stop solution (5 % phenol/95 % ethanol), cells pellets were immediately lysed with cell lysis buffer (85 mg/mL lysozyme in TE buffer, pH 8). One ml of Trizol reagent was added and samples were stored at -20C till the RNA isolation protocol was resumed. PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) characteristics strain background: MG1655 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) characteristics genotype/variation: phagePA8 in the argW gene PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) characteristics growth phase: exponentially growing cells PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) growth_protocol Overnight cultures were diluted till OD600 of 0.005 in LB medium (10 g/L tryptone, 5 g/L yeast extract, 5 g/L NaCl) and cells were grown at 37oC, 180 rpm to an OD600 of 0.4 - 0.6. PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) organism Escherichia coli K-12 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) source_name MG1655_φPA8 PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) treatment_protocol After the treatment with stop solution (5 % phenol/95 % ethanol), cells pellets were immediately lysed with cell lysis buffer (85 mg/mL lysozyme in TE buffer, pH 8). One ml of Trizol reagent was added and samples were stored at -20C till the RNA isolation protocol was resumed. PGCGROWTHCONDITIONS
SRR8587792 GSE126710 GSM3611674 GPL26204 PMID_31208335 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GPL26204: NextSeq 550 (Escherichia coli K-12) contact_name Petya,,Berger PGCGROWTHCONDITIONS