SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Illumina Casava1.7 software used for basecalling. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to E.coli K2 BW25113 whole genome using bowtie v0.12.2 PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Genome_build: E.coli K12 BW25113 PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	After chilling on ice, each 100-ml culture was mixed with 25 ml ice-cold ethanol/phenol solution (5% (v/v), water-saturated phenol in 95% (v/v) ethanol).  Cells were harvested by centrifugation at 7,000 g for 2 min at 4 °C, flash-frozen with dry ice, and stored at –80 °C. Two independent cultures for each sample were mixed together for determination of transcriptional profiles. Total RNA was extracted from the collected bacterial cells using TRIzol reagent (Thermo Fisher Scientifc). PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	rRNA was removed from total RNA by using the Ribo-Zero rRNA removal kit. Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Libraries were constructed using the Illumina Nextera XT kit and analyzed for concentration , size distribution and quantification of viable sequencing templates via qPCR. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	To generate Illumina-compatible sequencing libraries for each sample, molecular indexes were added to each library, allowing samples to be pooled and sequenced on the Illumina HiSeq 2500 with a 1 x 50 bp single-end configuration. More than 250 million reads were generated per sample, and at least 80% bases had quality scores above Q30. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	Overnight cultures of the dnaB-Ts single mutant (strain 2429) and the dnaB-Ts ΔahpC double mutant (strain 3780) were diluted 100-fold into fresh SB medium (3.2% peptone, 2% yeast extract and 1% NaCl), and they were then grown at 30 °C to early-log phase (OD600 = 0.15).  Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed, fresh SB medium, and they were grown at 30 °C for another 60 min. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	Bacterial cultures PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	treatment_protocol	Samples (200-ml) of the above cultures for each strain were split into two aliquots (each 100 ml), and the aliquots were then incubated at 30 °C or 42 °C for another 60 min. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	strain: 3780 PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	culture temperature: 42 °C for 60 min PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	medium: SB broth PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	Overnight cultures of the dnaB-Ts single mutant (strain 2429) and the dnaB-Ts ΔahpC double mutant (strain 3780) were diluted 100-fold into fresh SB medium (3.2% peptone, 2% yeast extract and 1% NaCl), and they were then grown at 30 °C to early-log phase (OD600 = 0.15).  Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed, fresh SB medium, and they were grown at 30 °C for another 60 min. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	Bacterial cultures PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	treatment_protocol	Samples (200-ml) of the above cultures for each strain were split into two aliquots (each 100 ml), and the aliquots were then incubated at 30 °C or 42 °C for another 60 min. PGCGROWTHCONDITIONS
SRR7140739	GSE114262	GSM3138598	GPL18133	PMID_30948634	dnaB-Ts_42°C	RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	contact_name	Yuzhi,,Hong PGCGROWTHCONDITIONS