GSE90056-GSM2396743-GPL15010-PMID_29183994.tsv 8.76 KB
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	Basecalls performed using Illumina CASAVA. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	Reads were trimmed and adaptors removed using the fastx_toolkit. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	Reads were aligned to the MG1655 genome with Bowtie. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	The number of reads at each position in the genome was counted using in-house Python scripts. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	The number of reads mapping to each gene was counted and normalized using EdgeR. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	Supplementary_files_format_and_content: ecoli_heatshock_rawcounts.txt: Tab-delimited text file has raw read counts per kilobase million for each E. coli gene (EcoCyc gene names). PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	data_processing	Supplementary_files_format_and_content: ecoli_heatshock_normalizedcounts.txt: Tab-delimited text file has EdgeR-normalized counts per kilobase million for each E. coli gene (EcoCyc gene names). PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	extract_protocol	Cells were lysed in liquid nitrogen, then samples were taken for total RNA. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	extract_protocol	Total RNA was extracted with phenol chloroform and alkaline fragmented, then fragments (20-40 nt) were gel-purified. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	extract_protocol	RNA was dephosphorylated, ligated to an adaptor, reverse transcribed, circularized then amplified with Illumina adaptors and barcodes. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	growth_protocol	E. coli were grown in defined rich media at 30C. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	organism	Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	source_name	E. coli cultures PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	treatment_protocol	Cells were induced with arabinose or heat shocked, then harvested and flash frozen. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	characteristics	plasmid: pBADsigma32I54N PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	characteristics	growth temperature: 30C PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	characteristics	induction: 0.2% arabinose PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	characteristics	treatment temperature: 30C PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	characteristics	heat shock time: None PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	characteristics	replicate: 2 PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	growth_protocol	E. coli were grown in defined rich media at 30C. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	organism	Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	source_name	E. coli cultures PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	treatment_protocol	Cells were induced with arabinose or heat shocked, then harvested and flash frozen. PGCGROWTHCONDITIONS
SRR5036091	GSE90056	GSM2396743	GPL15010	PMID_29183994	ecoli_k12_pBADsigma32I54N_30C_m_2	Translation efficiency is maintained during heat shock in Escherichia coli	GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655)	contact_name	Evan,Thomas,Powers PGCGROWTHCONDITIONS