GSE90056-GSM2396743-GPL15010-PMID_29183994.tsv
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SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing Basecalls performed using Illumina CASAVA. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing Reads were trimmed and adaptors removed using the fastx_toolkit. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing Reads were aligned to the MG1655 genome with Bowtie. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing The number of reads at each position in the genome was counted using in-house Python scripts. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing The number of reads mapping to each gene was counted and normalized using EdgeR. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing Supplementary_files_format_and_content: ecoli_heatshock_rawcounts.txt: Tab-delimited text file has raw read counts per kilobase million for each E. coli gene (EcoCyc gene names). PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) data_processing Supplementary_files_format_and_content: ecoli_heatshock_normalizedcounts.txt: Tab-delimited text file has EdgeR-normalized counts per kilobase million for each E. coli gene (EcoCyc gene names). PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) extract_protocol Cells were lysed in liquid nitrogen, then samples were taken for total RNA. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) extract_protocol Total RNA was extracted with phenol chloroform and alkaline fragmented, then fragments (20-40 nt) were gel-purified. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) extract_protocol RNA was dephosphorylated, ligated to an adaptor, reverse transcribed, circularized then amplified with Illumina adaptors and barcodes. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli were grown in defined rich media at 30C. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) source_name E. coli cultures PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) treatment_protocol Cells were induced with arabinose or heat shocked, then harvested and flash frozen. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) characteristics plasmid: pBADsigma32I54N PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) characteristics growth temperature: 30C PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) characteristics induction: 0.2% arabinose PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) characteristics treatment temperature: 30C PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) characteristics heat shock time: None PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) characteristics replicate: 2 PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli were grown in defined rich media at 30C. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) source_name E. coli cultures PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) treatment_protocol Cells were induced with arabinose or heat shocked, then harvested and flash frozen. PGCGROWTHCONDITIONS
SRR5036091 GSE90056 GSM2396743 GPL15010 PMID_29183994 ecoli_k12_pBADsigma32I54N_30C_m_2 Translation efficiency is maintained during heat shock in Escherichia coli GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) contact_name Evan,Thomas,Powers PGCGROWTHCONDITIONS