GSE9923-GSM247867-GPL199-GPL3154-PMID:18528414.tsv
1.18 KB
"RNA extracted from biofilm cells of E. coli K-12 BW25113 tnaA mutant after 7 hours of growth in LB with glasswool and 100 uM indole at 37ºC" "characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting" "data_processing.1"
"The overnight culture (2.5 ml) was used to inoculate 250 ml of fresh LB medium with 10 g of submerged glass wool (Corning Glass Works, Corning, NY) and with 100 uM indole for forming biofilm. After incubation for 7 h at 37°C with shaking (250 rpm), the glass wool was carefully and quickly removed and rinsed with 100 ml of sterile 0.85% NaCl solution at 0°C. Biofilm cells were removed by sonicating the glass wool in 200 ml of sterile 0.85% NaCl solution at 0°C. After breaking the cells with a bead beater, and the total RNA was isolated with Qiagen RNeasy mini Kit (Cat# 74104)" "extract_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Channel 1" "source_name_ch1.1"
"BW25113 tnaA Biofilm 100 uM indole 7h LB 37C" "title.1"
"RNA extracted from biofilm cells of E. coli K-12 BW25113 tnaA mutant after 7 hours of growth in LB with glasswool and 100 uM indole at 37ºC" "characteristics_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Channel 1" "source_name_ch1.1"