GSE9671-GSM244367-GPL6178-PMID:18725421.tsv
1.5 KB
"EDL933 infection of THP-1 cells, recovered 2h post-infection" "characteristics_ch1.1"
"EDL933 genomic DNA" "characteristics_ch2.1"
"After backgroud correction and total intensity normalization, ratio to reference were calculated and log base 2 transformed" "data_processing.1"
"TRIzol reagent (Gibco BRL) was used according to the manufacturer instruction. RNA was treated with DNase (Ambion). RNA sample was converted to cDNA in 5 independent reverse-transcription reactions as described previously (Graham, J. 1999. PNAS 96:11554-559). Bacterial transcript were purified from host transcript by using 3 round of the SCOTS procedure (Daigle, F. 2002. Methods Enzymol. 358:108-122)." "extract_protocol_ch1.1"
"Genomic DNA was extracted by standard phenol-chloroform method (Sambrook, J, Molecular Cloning, 3rd Edition)" "extract_protocol_ch2.1"
"EDL933 from overnight culture in LB broth" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"3XSCOTS cDNA obtained 2h post-infection" "source_name_ch1.1"
"Reference genomic DNA" "source_name_ch2.1"
"T2 THP-1 infection, Microarray #1 second replicate" "title.1"
"EDL933 infection of THP-1 cells, recovered 2h post-infection" "characteristics_ch1.1"
"EDL933 genomic DNA" "characteristics_ch2.1"
"EDL933 from overnight culture in LB broth" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"3XSCOTS cDNA obtained 2h post-infection" "source_name_ch1.1"
"Reference genomic DNA" "source_name_ch2.1"