GSE96551-GSM2535439-GPL14548-PMID:28363967.tsv 1.39 KB
"strain: BW25113"	"characteristics_ch1.1"
"product: Butanol"	"characteristics_ch1.2"
"Basecalls performed using CASAVA version 1.8.2"	"data_processing.1"
"Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to CP009273.1 whole genome using bowtie2"	"data_processing.2"
"Quantification of gene expression and analysis of gene differential expression were performed using Fragment Per Kilo bases per Million reads (FPKM) value based rsem software version 1.2.4 and edgeR version 3.4.2 (Bioconductor), respectively"	"data_processing.3"
"Genome_build: CP009273.1"	"data_processing.4"
"Supplementary_files_format_and_content: Excel file include FPKM values of different genes for each sample"	"data_processing.5"
"Total RNAs were isolated using TRIzol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used for the construction of sequencing libraries."	"extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols."	"extract_protocol_ch1.2"
"Escherichia coli"	"organism_ch1.1"
"Bacteria cells"	"source_name_ch1.1"
"MT_6h_rep2"	"title.1"
"RNA-Seq"	"library_strategy.1"
"strain: BW25113"	"characteristics_ch1.1"
"product: Butanol"	"characteristics_ch1.2"
"Escherichia coli"	"organism_ch1.1"
"Bacteria cells"	"source_name_ch1.1"