GSE94978-GSM2492646-GPL14548-PMID:28827616.tsv 1.67 KB
"strain: Poultry E. coli Virulent and multidrug resistant"	"characteristics_ch1.1"
"RNA-Seq of E. coli were done using blind and fit-only parameter in DE-Seq pakage"	"data_processing.1"
"Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence using FASTX-Toolkit  version 0.0.13 and Perl version 5.8.8"	"data_processing.2"
"first strand cDNA was performed by the use of SuperScriptII and the second strand cDNA synthesis was done before end-pair and dA-tailing"	"data_processing.3"
"Reads longer than 25 nt and ≤ 2 N (ambiguous nucleotides) were preserved."	"data_processing.4"
"Using blind and fit-only parameter in DE-Seq pakage, expressions of genes in all samples were changed to count per gene, using RNA-Seq protocol on Illumina HiSeq2500 platform"	"data_processing.5"
"Genome_build: UCSC mm10"	"data_processing.6"
"Supplementary_files_format_and_content: tab-delimited text files include normalized FPKM values and raw fragment counts for each Sample"	"data_processing.7"
"Total RNAs were extracted from the bacterial isolates using TRIzol"	"extract_protocol_ch1.1"
"Following \"TruSeq RNA Sample Preparation Guide\"."	"extract_protocol_ch1.2"
"Strains were harvested in MH broth at log phase"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Broiler Fecal"	"source_name_ch1.1"
"sp2"	"title.1"
"No treatment"	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"strain: Poultry E. coli Virulent and multidrug resistant"	"characteristics_ch1.1"
"Strains were harvested in MH broth at log phase"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Broiler Fecal"	"source_name_ch1.1"
"No treatment"	"treatment_protocol_ch1.1"