GSE92255-GSM2424647-GPL15982-PMID:.tsv 1.99 KB
"growth phase: Early exponential"	"characteristics_ch1.1"
"background: wild type, fis flag tag"	"characteristics_ch1.2"
"antibody: Monoclonal ANTI-FLAG M2 antibody (F3165) -Sigma"	"characteristics_ch1.3"
"Samples were aligned to the E. coli K 12 MG1655 reference sequence (version NC000913.2), using BWA (version 0.5) WITH -q 30 parameter for trimming of reads below PHRED quality score of 30."	"data_processing.1"
"SAM files thus obtained were converted to BAM files using samtools view command"	"data_processing.2"
"BAM files of each ChIP biological replicate and the input replicate samples were used in the MACS peak calling program with the -f BAMPE parameter"	"data_processing.3"
"Genome_build: NC000913.2"	"data_processing.4"
"Supplementary_files_format_and_content: peaks.bed"	"data_processing.5"
"Cells were fixed in the required growth phase in formaldehyde, quenched, washed and lysed. Fragmented DNA - protein complex were pulled using the monoclonal flag tag antibody. The complex was then washed, reverse cross-linked and obtained DNA purified using Qiagen min elute PCR purification kit."	"extract_protocol_ch1.1"
"Truseq ChIP kit was used for the library preparation. DNA was end repaired and adapters were ligated. After ligation, purify ligation products and then amplify."	"extract_protocol_ch1.2"
"Cells were inoculated from overnight culture in rich Lysogeny broth at 37 degrees in aerobic conditions."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"fis.ee.chipseq"	"source_name_ch1.1"
"fis.ee.chipseq"	"title.1"
"ChIP-Seq"	"library_strategy.1"
"growth phase: Early exponential"	"characteristics_ch1.1"
"background: wild type, fis flag tag"	"characteristics_ch1.2"
"antibody: Monoclonal ANTI-FLAG M2 antibody (F3165) -Sigma"	"characteristics_ch1.3"
"Cells were inoculated from overnight culture in rich Lysogeny broth at 37 degrees in aerobic conditions."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"fis.ee.chipseq"	"source_name_ch1.1"