GSE89507-GSM2374959-GPL22638-PMID:30013537.tsv 2.12 KB
"isolate: UR40"	"characteristics_ch1.1"
"treatment: none"	"characteristics_ch1.2"
"time point: 30min"	"characteristics_ch1.3"
"All data was processed using Rockhopper Ver.2.03. Rockhopper is an \"automatic\" bacterial RNA-seq analysis tool; for additional information see: McClure, R., et al. (2013). \"Computational analysis of bacterial RNA-Seq data.\" Nucleic Acids Res 41(14): e140. The default general parameters were used with the \"verbose output\" tab ticked and the \"orientation of mate-pair reads\" changed to \"rf\"."	"data_processing.1"
"Genome_build: NC_017644.1 (Escherichia coli NA114)"	"data_processing.2"
"Supplementary_files_format_and_content: *txt, *xml: These files contain the raw counts, normalized counts, RPKM values, expression values, P-values and Q-values. Each file contains the values for both samples at the indicated timepoint."	"data_processing.3"
"Total RNA was extracted using a Thermo Scientific GeneJET RNA isolation kit."	"extract_protocol_ch1.1"
"Extraction followed by DNase treatment with TURBO DNA-free kit from Ambion. rRNA depleted using an Illumina Ribo-zero rRNA removal kit. Followed by RNA-seq library prep using an Illumina TruSeq Stranded mRNA Library Prep Kit."	"extract_protocol_ch1.2"
"The cultures were balanced and grown in CAMHB media at 37 degrees Celsius."	"growth_protocol_ch1.1"
"Escherichia coli O25b:H4-ST131"	"organism_ch1.1"
"Clinical isolate, minus ciprofloxacin, 30min"	"source_name_ch1.1"
"30min minus ciprofloxacin"	"title.1"
"E. coli ST131 UR40 was treated with ciprofloxacin (2µg/mL) and samples were taken at time points 0 min, 30 min and 90min."	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"isolate: UR40"	"characteristics_ch1.1"
"treatment: none"	"characteristics_ch1.2"
"time point: 30min"	"characteristics_ch1.3"
"The cultures were balanced and grown in CAMHB media at 37 degrees Celsius."	"growth_protocol_ch1.1"
"Escherichia coli O25b:H4-ST131"	"organism_ch1.1"
"Clinical isolate, minus ciprofloxacin, 30min"	"source_name_ch1.1"
"E. coli ST131 UR40 was treated with ciprofloxacin (2µg/mL) and samples were taken at time points 0 min, 30 min and 90min."	"treatment_protocol_ch1.1"