GSE87856-GSM2341961-GPL14548-PMID:28115545.tsv 1.84 KB
"strain: BW27786 (K-12 derivative)"	"characteristics_ch1.1"
"rpos level: 100%"	"characteristics_ch1.2"
"Base calls performed with RTA v1.18.5"	"data_processing.1"
"the first ten base pairs of each read were trimmed using FASTX­Toolkit 0.0.13."	"data_processing.2"
"Reads were mapped using BWA 0.7.5a"	"data_processing.3"
"The number read pairs mapped to each gene was counted with HTSeq 0.6.1"	"data_processing.4"
"Supplementary_files_format_and_content: A tab deliminited file with counts for each gene."	"data_processing.5"
"RNA was purified from 200 µL of overnight culture by pelleting and resuspeding in 500 µL of Trizol at 65°C, followed by purification on a column (Direct-Zol, Zymo Research). Samples received two 30-minute DNase treatments using TURBO DNA-free (Ambion) following the manufacturers instructions. RNA samples were then purified on a column (RNA Clean & Concentrator, Zymo Research). Three samples were prepped from each culture and pooled to generate sufficient RNA."	"extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols"	"extract_protocol_ch1.2"
"Cells were inoculated from frozen cultures into 5 mL of LB, and grown.  5 µL of this overnight culture was diluted into 5 mL of LB with the appropriate concentration of arabinose, and grown for 20h."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli culture"	"source_name_ch1.1"
"100% rep1"	"title.1"
"RNA-Seq"	"library_strategy.1"
"strain: BW27786 (K-12 derivative)"	"characteristics_ch1.1"
"rpos level: 100%"	"characteristics_ch1.2"
"Cells were inoculated from frozen cultures into 5 mL of LB, and grown.  5 µL of this overnight culture was diluted into 5 mL of LB with the appropriate concentration of arabinose, and grown for 20h."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli culture"	"source_name_ch1.1"