GSE8708-GSM215707-GPL199-PMID:18545668.tsv 1.09 KB
"RNA extracted from biofilm cells of E. coli K-12 wild type after 4 hours of growth at 37C in LB with glasswool."	"characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"“The overnight culture (2.5 ml) was used to inoculate 250 ml of fresh LB medium with 10 g of submerged glass wool (Corning Glass Works, Corning, NY) for forming biofilm. After incubation for 15 h at 37°C with shaking (250 rpm), the glass wool was carefully and quickly removed and rinsed with 100 ml of sterile 0.85% NaCl solution at 0°C. Biofilm cells were removed by sonicating the glass wool in 200 ml of sterile 0.85% NaCl solution at 0°C. After breaking the cells with a bead beater, and the total RNA was isolated with Qiagen RNeasy mini Kit (Cat# 74104)."	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Channel 1"	"source_name_ch1.1"
"BW25113 w/t LB 4hr biofilm cells"	"title.1"
"RNA extracted from biofilm cells of E. coli K-12 wild type after 4 hours of growth at 37C in LB with glasswool."	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Channel 1"	"source_name_ch1.1"