GSE80827-GSM2138444-GPL13359-PMID:28013396.tsv
1.86 KB
"strain: TB1" "characteristics_ch1.1"
"Raw Microarray data evaluation including preprocessing, normalization, grouping the differentially expressed genes and statistical analyses with Moderated T-test and Bonferroni FWER was carried out with GeneSpring 13.0 Software." "data_processing.1"
"Total RNA extraction was carried out based on the protocols given in the manual of QIAGEN - RNAprotect Bacteria Reagent Handbook, QIAGEN - RNeasy Mini Kit and QIAGEN - RNase-free DNase set. The concentration of each RNA sample was measured by Qubit® RNA BR Assay Kit (Invitrogen)." "extract_protocol_ch1.1"
"The pre-culture was the overnight propagated cells in 5 ml of LB medium at 37ºC and 180 rpm. The main bacterial culture was prepared by 1% inoculation from pre-culture when optical densities at 600 nm (i.e., OD600) reached 0.7. Growth was achieved in 50 ml of LB medium." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"DMSO treatment, 0 min, replicate 1" "source_name_ch1.1"
"DMSO_0min_rep1" "title.1"
"When OD600 of the culture reached 0.54±0.06, cells were treated with 100 µg/ml of (-)-Roemerine. Since the alkaloid was dissolved in DMSO, control cells were also treated with the same amount of DMSO." "treatment_protocol_ch1.1"
"strain: TB1" "characteristics_ch1.1"
"The pre-culture was the overnight propagated cells in 5 ml of LB medium at 37ºC and 180 rpm. The main bacterial culture was prepared by 1% inoculation from pre-culture when optical densities at 600 nm (i.e., OD600) reached 0.7. Growth was achieved in 50 ml of LB medium." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"DMSO treatment, 0 min, replicate 1" "source_name_ch1.1"
"When OD600 of the culture reached 0.54±0.06, cells were treated with 100 µg/ml of (-)-Roemerine. Since the alkaloid was dissolved in DMSO, control cells were also treated with the same amount of DMSO." "treatment_protocol_ch1.1"