GSE6992-GSM161214-GPL3154-PMID:18000553.tsv
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"Escherichia coli MG1655 2 minutes paraquat treatment" "characteristics_ch1.1"
"The data were analyzed using Robust Multi-Array average as implemented in Bioconductor" "data_processing.1"
"Total RNA was isolated using Qiagen RNeasy Mini Kit and treated with DNase I" "extract_protocol_ch1.1"
"Overnight cultures were inoculated 1:100 into 20ml of EZ Rich Defined Medium (Neidhardt et al., 1974) in 125 ml Erlenmeyer flasks and grown at 37oC and 250 rpm in a reciprocating water bath." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli MG1655 2 minutes paraquat treatment" "source_name_ch1.1"
"M1655 wild type at t2, rep2" "title.1"
"Paraquat was added to growing cultures at a final concentration of 250 ?M. Samples (1.4ml) were taken every 2 minutes following paraquat treatment for the length of the time course, and flash-frozen by immersion of the tubes in liquid nitrogen. The cells were collected by centrifugation for 1 minute at 13,000 rpm," "treatment_protocol_ch1.1"
"Escherichia coli MG1655 2 minutes paraquat treatment" "characteristics_ch1.1"
"Overnight cultures were inoculated 1:100 into 20ml of EZ Rich Defined Medium (Neidhardt et al., 1974) in 125 ml Erlenmeyer flasks and grown at 37oC and 250 rpm in a reciprocating water bath." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli MG1655 2 minutes paraquat treatment" "source_name_ch1.1"
"Paraquat was added to growing cultures at a final concentration of 250 ?M. Samples (1.4ml) were taken every 2 minutes following paraquat treatment for the length of the time course, and flash-frozen by immersion of the tubes in liquid nitrogen. The cells were collected by centrifugation for 1 minute at 13,000 rpm," "treatment_protocol_ch1.1"