GSE6992-GSM161209-GPL3154-PMID:18000553.tsv 1.72 KB
"Escherichia coli MG1655 4 minutes paraquat treatment"	"characteristics_ch1.1"
"The data were analyzed using Robust Multi-Array average as implemented in Bioconductor"	"data_processing.1"
"Total RNA was isolated using Qiagen RNeasy Mini Kit and treated with DNase I"	"extract_protocol_ch1.1"
"Overnight cultures were inoculated 1:100 into 20ml of EZ Rich Defined Medium (Neidhardt et al., 1974) in 125 ml Erlenmeyer flasks and grown at 37oC and 250 rpm in a reciprocating water bath."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli MG1655 4 minutes paraquat treatment"	"source_name_ch1.1"
"M1655 wild type at t4, rep1"	"title.1"
"Paraquat was added to growing cultures at a final concentration of 250 ?M. Samples (1.4ml) were taken every 2 minutes following paraquat treatment for the length of the time course, and flash-frozen by immersion of the tubes in liquid nitrogen.  The cells were collected by centrifugation for 1 minute at 13,000 rpm,"	"treatment_protocol_ch1.1"
"Escherichia coli MG1655 4 minutes paraquat treatment"	"characteristics_ch1.1"
"Overnight cultures were inoculated 1:100 into 20ml of EZ Rich Defined Medium (Neidhardt et al., 1974) in 125 ml Erlenmeyer flasks and grown at 37oC and 250 rpm in a reciprocating water bath."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli MG1655 4 minutes paraquat treatment"	"source_name_ch1.1"
"Paraquat was added to growing cultures at a final concentration of 250 ?M. Samples (1.4ml) were taken every 2 minutes following paraquat treatment for the length of the time course, and flash-frozen by immersion of the tubes in liquid nitrogen.  The cells were collected by centrifugation for 1 minute at 13,000 rpm,"	"treatment_protocol_ch1.1"