GSE69912-GSM1712366-GPL8850-PMID:.tsv 1.89 KB
"strain background: BW25113"	"characteristics_ch1.1"
"genotype/variation: DnaK deletant"	"characteristics_ch1.2"
"strain background: BW25113"	"characteristics_ch2.1"
"genotype/variation: wild type"	"characteristics_ch2.2"
"The signal/noise ratio for each array was calculated, for each spot and each channel, as (foreground intensity minus background intensity)/(standard deviation of the background). The raw data were normalized  using a space and intensity-dependant LOWESS program. Faint spots were removed from the data if the intensities were lower than the average intensity plus 2 standard deviations of the negative controls. The significant genes differentiating wild-type and deleting dnaK were selected based on q<0.05 and greater than twofold as criteria."	"data_processing.1"
"Total RNA was isolated from strains using NucleoSpin RNAII kit (Macherey-Nagel Corporation) following the instruction. RNA samples were examined in a 1.5% denaturing agarose gel, quantitated by absorbance at 260 nm and stored until further use for microarray."	"extract_protocol_ch1.1"
"Total RNA was isolated from strains using NucleoSpin RNAII kit (Macherey-Nagel Corporation) following the instruction. RNA samples were examined in a 1.5% denaturing agarose gel, quantitated by absorbance at 260 nm and stored until further use for microarray."	"extract_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"DnaK deletant_37C"	"source_name_ch1.1"
"WT_37C"	"source_name_ch2.1"
"E.coli gene expression data in deletion of chaperone DnaK"	"title.1"
"strain background: BW25113"	"characteristics_ch1.1"
"genotype/variation: DnaK deletant"	"characteristics_ch1.2"
"strain background: BW25113"	"characteristics_ch2.1"
"genotype/variation: wild type"	"characteristics_ch2.2"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"DnaK deletant_37C"	"source_name_ch1.1"
"WT_37C"	"source_name_ch2.1"