GSE69912-GSM1712366-GPL8850-PMID:.tsv
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"strain background: BW25113" "characteristics_ch1.1"
"genotype/variation: DnaK deletant" "characteristics_ch1.2"
"strain background: BW25113" "characteristics_ch2.1"
"genotype/variation: wild type" "characteristics_ch2.2"
"The signal/noise ratio for each array was calculated, for each spot and each channel, as (foreground intensity minus background intensity)/(standard deviation of the background). The raw data were normalized using a space and intensity-dependant LOWESS program. Faint spots were removed from the data if the intensities were lower than the average intensity plus 2 standard deviations of the negative controls. The significant genes differentiating wild-type and deleting dnaK were selected based on q<0.05 and greater than twofold as criteria." "data_processing.1"
"Total RNA was isolated from strains using NucleoSpin RNAII kit (Macherey-Nagel Corporation) following the instruction. RNA samples were examined in a 1.5% denaturing agarose gel, quantitated by absorbance at 260 nm and stored until further use for microarray." "extract_protocol_ch1.1"
"Total RNA was isolated from strains using NucleoSpin RNAII kit (Macherey-Nagel Corporation) following the instruction. RNA samples were examined in a 1.5% denaturing agarose gel, quantitated by absorbance at 260 nm and stored until further use for microarray." "extract_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"DnaK deletant_37C" "source_name_ch1.1"
"WT_37C" "source_name_ch2.1"
"E.coli gene expression data in deletion of chaperone DnaK" "title.1"
"strain background: BW25113" "characteristics_ch1.1"
"genotype/variation: DnaK deletant" "characteristics_ch1.2"
"strain background: BW25113" "characteristics_ch2.1"
"genotype/variation: wild type" "characteristics_ch2.2"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"DnaK deletant_37C" "source_name_ch1.1"
"WT_37C" "source_name_ch2.1"