GSE69829-GSM1709736-GPL20322-PMID:28348816.tsv
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"strain: CFT073 nsrR::3X-Flag tag" "characteristics_ch1.1"
"chip antibody: Monoclonal ANTI-FLAG M5(Sigma-Aldrich, catalog number: F4042 )" "characteristics_ch1.2"
"ChIP-seq reads were aligned to the CFT073 genome using bowtie (version 0.12.8)" "data_processing.1"
"peaks were called using MACS2 with default parameters" "data_processing.2"
"Genome_build: CFT073(AE014075.1)" "data_processing.3"
"Supplementary_files_format_and_content: bed format peak file generated from MACS2" "data_processing.4"
"Cells were disrupted by sonication and NsrR bound DNA was isolated with anti-Flag antibody." "extract_protocol_ch1.1"
"DNA fragments were treated with Epicentre End-It DNA End Repair Kit and 3’ A overhangs were added with DNA polymerase I (Klenow fragment). Adapters from the IlluminaTruSeq DNA sample preparation kit were ligated using LigFast (Promega) and DNAs were amplified by PCR using primers provided in the IlluminaTruSeq DNA sample preparation kit and Phusion DNA polymerase (NEB). Products of the ligation reaction and PCR amplification in the range 300-400 bp were purified by 2% agarose gel electrophoresis. DNA concentrations were measured using Qubit™ dsDNA HS Assay Kits (Invitrogen)." "extract_protocol_ch1.2"
"samples were grown aerobically in rich media" "growth_protocol_ch1.1"
"Escherichia coli CFT073" "organism_ch1.1"
"uropathogenic Escherichia coli (UPEC)" "source_name_ch1.1"
"NsrR_ChIPSeq_rep3" "title.1"
"ChIP-Seq" "library_strategy.1"
"strain: CFT073 nsrR::3X-Flag tag" "characteristics_ch1.1"
"chip antibody: Monoclonal ANTI-FLAG M5(Sigma-Aldrich, catalog number: F4042 )" "characteristics_ch1.2"
"samples were grown aerobically in rich media" "growth_protocol_ch1.1"
"Escherichia coli CFT073" "organism_ch1.1"
"uropathogenic Escherichia coli (UPEC)" "source_name_ch1.1"