"strain: MG1655"	"characteristics_ch1.1"
"genotype/variation: WT"	"characteristics_ch1.2"
"agent: glycolaldehyde"	"characteristics_ch1.3"
"Data were analyzed by Feature Extraction V.11.5.1.1. RNA"	"data_processing.1"
"The RNeasy Mini Kit (QIAGEN) was used to extract RNA. Quantity and quality of the samples were determined by NanoDrop (Thermo) and Bioanalyzer (Agilent Technologies), respectively"	"extract_protocol_ch1.1"
"M9 mineral medium containing (D)-xylose as the only carbon source (100 ml in 500 ml shake flasks) was inoculated from exponentially growing wild-type cells or synthetic cells (cultivated on xylose M9 medium) to adjust an OD of ~0.1. Cultures were incubated at 37°C under shaking until OD reached ~1. Then they were split into two 50 ml aliquots and further cultivated in 250 ml shake flasks in the presence or absence of 10 mM glycolaldehyde. After 30 min of incubation, 1 ml of the cell suspension was withdrawn and centrifuged at 1500 x g (Eppendorf 5415D) for 5 min. The supernatant was removed and the cell pellets were directly subject to RNA extraction. Experiment were repeated three times."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"wild-type MG1655 + glycolaldehyde 10 mM"	"source_name_ch1.1"
"WT strain treated with glycolaldehyde first repetition"	"title.1"
"WT cells were treated with 10 mM Glycolaldehyde for 30 min whenever the culture OD600nm reached ~1."	"treatment_protocol_ch1.1"
"strain: MG1655"	"characteristics_ch1.1"
"genotype/variation: WT"	"characteristics_ch1.2"
"agent: glycolaldehyde"	"characteristics_ch1.3"
"M9 mineral medium containing (D)-xylose as the only carbon source (100 ml in 500 ml shake flasks) was inoculated from exponentially growing wild-type cells or synthetic cells (cultivated on xylose M9 medium) to adjust an OD of ~0.1. Cultures were incubated at 37°C under shaking until OD reached ~1. Then they were split into two 50 ml aliquots and further cultivated in 250 ml shake flasks in the presence or absence of 10 mM glycolaldehyde. After 30 min of incubation, 1 ml of the cell suspension was withdrawn and centrifuged at 1500 x g (Eppendorf 5415D) for 5 min. The supernatant was removed and the cell pellets were directly subject to RNA extraction. Experiment were repeated three times."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"wild-type MG1655 + glycolaldehyde 10 mM"	"source_name_ch1.1"
"WT cells were treated with 10 mM Glycolaldehyde for 30 min whenever the culture OD600nm reached ~1."	"treatment_protocol_ch1.1"