GSE6925-GSM159472-GPL199-PMID:17765265.tsv
1004 Bytes
"RNA extracted from biofilm cells of BW25113 ymgB mutant after 24h of growth in LBglu with glass wool" "characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting" "data_processing.1"
"To lyse the cells, 1.0 mL RLT buffer (Qiagen, Inc., Valencia, CA) and 0.2 mL 0.1 mm zirconia/silica beads (Biospec) were added to the frozen bead beater tubes containing the cell pellets. The tubes were closed tightly and beat for 50 seconds at the maximum speed in a mini bead beater (cat. no. 3110BX, Biospec). The total RNA was isolated by following the protocol of the RNeasy Mini Kit (Qiagen) including an on-column DNase digestion with RNase-free DNase I (Qiagen). " "extract_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Channel 1" "source_name_ch1.1"
"BW25113 ymgB LBglu 24h biofilm cells" "title.1"
"RNA extracted from biofilm cells of BW25113 ymgB mutant after 24h of growth in LBglu with glass wool" "characteristics_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Channel 1" "source_name_ch1.1"