GSE6781-GSM156734-GPL3051-PMID:17449618.tsv 2.2 KB
"Strain: MG1655 pGIT1 (carries the ytfE promoter in multicopy, titrates out NsrR to phenocopy an NsrR- mutation) Growth: Anaerobic to early exponential growth Media:Minimal salts + 20 mM trimethylamine-N-oxide +2.5 mM sodium nitrite"	"characteristics_ch1.1"
"Strain: MG1655 pGIT8 (carries 1 base pair deletion in the NsrR-binding site of the ytfE promoter) Growth: Anaerobic to early exponential growth Media:Minimal salts + 20 mM trimethylamine-N-oxide Pool of eight independent cultures"	"characteristics_ch2.1"
"Data was analysed using GeneSpring"	"data_processing.1"
"An RNeasy midikit was used to prepare total RNA according to the manufacturer’s instructions (QIAGEN Ltd.). Any contaminating DNA was removed using a DNAase column kit (QIAGEN Ltd)."	"extract_protocol_ch1.1"
"An RNeasy midikit was used to prepare total RNA according to the manufacturer’s instructions (QIAGEN Ltd.). Any contaminating DNA was removed using a DNAase column kit (QIAGEN Ltd)."	"extract_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"pGIT1 + NO2"	"source_name_ch1.1"
"pGIT8"	"source_name_ch2.1"
"pGIT1.4 + NO2"	"title.1"
"15 ml of culture was mixed with 30 ml of RNAprotect bacterial reagent (QIAGEN Ltd)."	"treatment_protocol_ch1.1"
"15 ml of culture was mixed with 30 ml of RNAprotect bacterial reagent (QIAGEN Ltd)."	"treatment_protocol_ch2.1"
"Strain: MG1655 pGIT1 (carries the ytfE promoter in multicopy, titrates out NsrR to phenocopy an NsrR- mutation) Growth: Anaerobic to early exponential growth Media:Minimal salts + 20 mM trimethylamine-N-oxide +2.5 mM sodium nitrite"	"characteristics_ch1.1"
"Strain: MG1655 pGIT8 (carries 1 base pair deletion in the NsrR-binding site of the ytfE promoter) Growth: Anaerobic to early exponential growth Media:Minimal salts + 20 mM trimethylamine-N-oxide Pool of eight independent cultures"	"characteristics_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"pGIT1 + NO2"	"source_name_ch1.1"
"pGIT8"	"source_name_ch2.1"
"15 ml of culture was mixed with 30 ml of RNAprotect bacterial reagent (QIAGEN Ltd)."	"treatment_protocol_ch1.1"
"15 ml of culture was mixed with 30 ml of RNAprotect bacterial reagent (QIAGEN Ltd)."	"treatment_protocol_ch2.1"