GSE66441-GSM1633277-GPL17439-PMID:26258987.tsv
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"strain: K-12 MG1655" "characteristics_ch1.1"
"genotype: WT" "characteristics_ch1.2"
"chip antibody: anti-RpoS (neoclone, WP009)" "characteristics_ch1.3"
"ChIP-exo reads were aligned to the ASM584v2 genome reference sequence using using bowtie v1.0.0 with parameters -S" "data_processing.1"
"MACE software (https://code.google.com/p/chip-exo/) was used to detect peaks with aligned output from bowtie mapping." "data_processing.2"
"Read count was calculated for each genomic position from sequence alignment, and 95% strongest intensity was used as background intensity." "data_processing.3"
"For each peak detected with MACE, binding intensity was calculated by averaging read counts from two biological replicates and dividing by background intensity." "data_processing.4"
"Genome_build: ASM584v2" "data_processing.5"
"Supplementary_files_format_and_content: Tab-delimited text files in gff format which has 8 columns: sequence id, source(empty), feature (+/- strand), start position, end position, intensity score, strand(+/-), frame(.), attribute(.)." "data_processing.6"
"ChIP-seq libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.1"
"E. coli K-12 MG1655 WT, GadE-8-myc, GadW-8-myc, and GadX-8-myc tagged strains were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Immunoprecipitated DNA" "source_name_ch1.1"
"ChIP-exo RpoS pH5.5 1" "title.1"
"ChIP-Seq" "library_strategy.1"
"strain: K-12 MG1655" "characteristics_ch1.1"
"genotype: WT" "characteristics_ch1.2"
"chip antibody: anti-RpoS (neoclone, WP009)" "characteristics_ch1.3"
"E. coli K-12 MG1655 WT, GadE-8-myc, GadW-8-myc, and GadX-8-myc tagged strains were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Immunoprecipitated DNA" "source_name_ch1.1"